RNA and dsRNA

RNA oligonucleotides can be synthesized by different strategies, which differ by the choice of protection of the 2'-hydroxyl group on the ribose. We offer 2'-O-Methyl or 2'-O-Silyl RNA synthesis.

O-Methyl RNA Oligonucleotide Synthesis

The methyl moiety at the 2'-hydroxyl is not removed prior to use resulting in nuclease resistance.

Possible modifications:

  • 2'-OMe-T
  • 2'-OMe-I
  • 2'-OMe-5-Me-C
  • 2'-OMe-2-amino-A
  • 2'-OMe-2-aminopurine
  • 2'-OMe-5-Br-U

These modifications are not allowed in the 3' position.

O-Silyl RNA Oligonucleotide Synthesis

The most common strategy to produce ordinary RNA oligonucleotides is by 2'-protection with O-Silyl. Post synthesis, the oligonucleotides are deprotected (desilylated) and desalted.

Possible modifications:

  • 5-Me-U (T)
  • Inosine
  • 5-Me-C
  • 5-Br-U
  • 5-I-U
  • 2-Amino-A
  • 2-Aminopurine

These modifications are not allowed in the 3' position.


We also synthesize custom defined double stranded RNA with 3'-dTdT and 2X HPLC purified.

Send us an email  for more information.