RNA and dsRNA
RNA oligonucleotides can be synthesized by different strategies, which differ by the choice of protection of the 2'-hydroxyl group on the ribose. We offer 2'-O-Methyl or 2'-O-Silyl RNA synthesis.
O-Methyl RNA Oligonucleotide Synthesis
The methyl moiety at the 2'-hydroxyl is not removed prior to use resulting in nuclease resistance.
Possible modifications:
- 2'-OMe-T
- 2'-OMe-I
- 2'-OMe-5-Me-C
- 2'-OMe-2-amino-A
- 2'-OMe-2-aminopurine
- 2'-OMe-5-Br-U
These modifications are not allowed in the 3' position.
O-Silyl RNA Oligonucleotide Synthesis
The most common strategy to produce ordinary RNA oligonucleotides is by 2'-protection with O-Silyl. Post synthesis, the oligonucleotides are deprotected (desilylated) and desalted.
Possible modifications:
- 5-Me-U (T)
- Inosine
- 5-Me-C
- 5-Br-U
- 5-I-U
- 2-Amino-A
- 2-Aminopurine
These modifications are not allowed in the 3' position.
dsRNA
We also synthesize custom defined double stranded RNA with 3'-dTdT and 2X HPLC purified.
Send us an email for more information.